Azotobacter vinelandii Ribonucleic Acid Polymerase

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Azotobacter vinehndii Ribonucleic Acid Polymerase

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Azotobacter vinehndii Ribonucleic Acid Polymerase III. RIBONUCLEIC ACID CI-IAI;“\; IXITIATION*

RNA synthesis by the Azofobacter vinelandii RNA polymerase is initiated with the incorporation of a ribonucleoside triphosphate to form the 5’ terminus of the chain. The RNA chains synthesized in vifro are preferentially initiated by purine ribonucleoside triphosphates. The ratio of guanosine triphosphate to ATP termini is determined by the DNA used to direct the synthesis of RNA. When denature...

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Genes in Azotobacter vinelandii

Azotobacter vinelandii contains a heterodimeric, membrane-bound [NiFelhydrogenase capable of catalyzing the reversible oxidation of H2. The 13 and at subunits of the enzyme are encoded by the structural genes hoxK and hoxG, respectively, which appear to form part of an operon that contains at least one further potential gene (open reading frame 3 1ORF3]). In this study, determination of the nuc...

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Plasmids of Azotobacter vinelandii.

Four laboratory strains and two isolates of Azotobacter vinelandii were found to contain plasmids. Twenty-five laboratory strains which could fix nitrogen did not have free, covalently closed circular plasmid DNA. The plasmids varied in size from 9 to 52 megadaltons, and each strain yielded only one plasmid. No discernible differences in ability to fix nitrogen were found between plasmid-bearin...

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Ultrastructure of Azotobacter vinelandii.

Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not reported previously: intine fibrils, intine vesicles, intine membrane, and mi...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1969

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)63570-7